Replicative Helicases: A Staircase with a Twist
نویسنده
چکیده
The first crystal structure of a ring helicase encircling single-stranded DNA reveals a mechanism for ATP-dependent DNA translocation.
منابع مشابه
The Hexameric Helicase DnaB Adopts a Nonplanar Conformation during Translocation
DNA polymerases can only synthesize nascent DNA from single-stranded DNA (ssDNA) templates. In bacteria, the unwinding of parental duplex DNA is carried out by the replicative DNA helicase (DnaB) that couples NTP hydrolysis to 5' to 3' translocation. The crystal structure of the DnaB hexamer in complex with GDP-AlF(4) and ssDNA reported here reveals that DnaB adopts a closed spiral staircase qu...
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DNA helicases are proteins that use the energy of NTP hydrolysis to processively denature duplex DNA.' As such, helicases can be considered similar to other mechanochemical motors that can catalyze directional movement along a polymeric lattice (e.g., microtubule-based motors as kinesin). Acting during DNA replication, helicases convert the duplex DNA to single strands and thereby activate the ...
متن کاملThe Balance between Recombination Enzymes and Accessory Replicative Helicases in Facilitating Genome Duplication
Accessory replicative helicases aid the primary replicative helicase in duplicating protein-bound DNA, especially transcribed DNA. Recombination enzymes also aid genome duplication by facilitating the repair of DNA lesions via strand exchange and also processing of blocked fork DNA to generate structures onto which the replisome can be reloaded. There is significant interplay between accessory ...
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DNA-protein cross-links (DPCs) are formed when cells are exposed to various DNA-damaging agents. Because DPCs are extremely large, steric hindrance conferred by DPCs is likely to affect many aspects of DNA transactions. In DNA replication, DPCs are first encountered by the replicative helicase that moves at the head of the replisome. However, little is known about how replicative helicases resp...
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DNA helicases unwind or rearrange duplex DNA during replication, recombination and repair. Helicases of many pathogenic organisms such as viruses, bacteria, and protozoa have been studied as potential therapeutic targets to treat infectious diseases, and human DNA helicases as potential targets for anti-cancer therapy. DNA replication machineries perform essential tasks duplicating genome in ev...
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ورودعنوان ژورنال:
- Current Biology
دوره 16 شماره
صفحات -
تاریخ انتشار 2006